The method of claim 1, wherein said method further comprises the step of introducing into the host cell one or more additional nucleic acids encoding one or more enzymes selected from the group consisting of glycosyltransferases, glycosidases and sugar transporters.ġ8. The method of claim 1, wherein the method further comprises culturing said host cell in the presence of a sialic acid donor or a precursor of a sialic acid donor.ġ7. The method of claim 1, wherein said host cell comprises a cellular pool of CMP-sialic acid.ġ6.
A method for producing a recombinant sialylated glycoprotein in a host cell, the host cell selected or engineered to produce glycoproteins comprising a GalGlcNAcMan 5GlcNAc 2 glycoform, the method comprising the step of introducing into the host cell a nucleic acid encoding an enzyme having sialyltransferase activity, the enzyme comprising a sialyltransferase catalytic domain and a cellular targeting signal peptide to target the sialyltransferase catalytic domain to the secretory pathway of the host cell wherein, upon passage of the recombinant glycoprotein through the secretory pathway of the host cell, a recombinant glycoprotein comprising a NANAGalGlcNAcMan 5GlcNA 2 glycoform is produced.ġ2. The method of claim 6, wherein said method further comprises the step of introducing into the host cell one or more nucleic acids encoding one or more enzymes involved in the biosynthesis or transport of CMP-Sialic acid.ġ0. The method of claim 6, wherein said nucleic acid encodes a fusion protein comprising a trans-sialidase catalytic domain with a cellular targeting signal peptide to target the trans-sialidase catalytic domain to the secretory pathway, the cell wall or the cell membrane of the host cell.Ĩ. A method for producing a recombinant sialylated glycoprotein in a eukaryotic host cell comprising introducing into the host cell a nucleic acid encoding a trans-sialidase enzyme.ħ. The method of claim 1, wherein the method further comprises the step of introducing into the host cell a nucleic acid encoding one or more enzymes involved in the biosynthesis or transport of CMP-Sia.Ħ. The method of claim 1, wherein the cellular targeting signal peptide targets the sialyltransferase catalytic domain to a location in the secretory pathway selected from the group consisting of the endoplasmic reticulum, the Golgi apparatus, the trans-Golgi network and secretory vesicles.ģ. A method for producing a recombinant sialylated glycoprotein in a host cell, the host cell selected or engineered to produce glycoproteins comprising a Gal (1-4)GlcNAc (1-4)Man 3GlcNAc 2 glycoform, the method comprising the step of introducing into the host cell a nucleic acid encoding an enzyme having sialyltransferase activity, the enzyme comprising a sialyltransferase catalytic domain and a cellular targeting signal peptide to target the sialyltransferase catalytic domain to the secretory pathway of the host cell wherein, upon passage of the recombinant glycoprotein through the secretory pathway of the host cell, a recombinant glycoprotein comprising a NANA (1-4)Gal (1-4)GlcNAc (1-4)Man 3GlcNA 2 glycoform is produced.Ģ. Sample depositing device for a cell sorterġ. Identification of Crmp4 as a Convergent Regulator of Axon Outgrowth InhibitionĬOMPOSITION AND BIOREMEDIATION METHOD FOR WATER POLLUTED BY HYDROCARBONS PHOSPHADIAZINE HCV POLYMERASE INHIBITORS III AND VI Heterologus expression of trypanothione reductase from Leishmania donovani in a prokaryotic system Method for Chondrocyte Expansion with Phenotype RetentionĬompositions, methods and systems for preparation of a stem cell-enriched cell population
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